| ORIGINAL ARTICLES |
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| Year : 2023 | Volume
: 7
| Issue : 1 | Page : 26-32 |
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Effects of Hibiscus sabdariffa L. extract on multispecies Porphyromonas gingivalis and Treponema denticola biofilms in vitro
Angela Winson1, Trijani Suwandi2, Olivia Nauli Komala2
1 Faculty of Dentistry, Universitas Trisakti, Jakarta, Indonesia
2 Department of Periodontology, Faculty of Dentistry, Universitas Trisakti, Jakarta, Indonesia
Correspondence Address:
Trijani Suwandi
Department of Periodontology, Faculty of Dentistry, Universitas Trisakti, Kyai Tapa No. 260, West Jakarta
Indonesia
 Source of Support: None, Conflict of Interest: None
DOI: 10.4103/SDJ.SDJ_4_23
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Background: Porphyromonas gingivalis and Treponema denticola are bacteria that play a crucial role in periodontitis. Hibiscus sabdariffa L. contains flavonoids, alkaloids, saponins, and tannins; compounds which are expected to inhibit biofilm formation with minimal side effects. Objectives: To determine the secondary metabolite compounds present in H. sabdariffa L. and its effect on multispecies biofilms formation consisted of P. gingivalis and T. denticola. Methods: This study used H. sabdariffa L. calyx extract with concentrations of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, and 1.5625%. The positive control used in this study was 0.2% chlorhexidine, and the negative control was brain heart infusion broth. The bacterial biofilm was grown for 1, 6, and 24 hours. Results: Hibiscus sabdariffa L. 1.5625% extracted in a 1-h incubation period, 6.25% extracted in a 6-h incubation period, and 3.25% extracted in a 24-h incubation period are more effective than chlorhexidine 0.2% in inhibiting multispecies P. gingivalis and T. denticola biofilms (P < 0.05). Conclusion: Hibiscus sabdariffa L. calyx extract significantly inhibits multispecies P. gingivalis and T. denticola biofilm with the most effective concentration at 100% at 1-h, 6-h, and 24-h incubation periods. Future studies are needed to test the toxicity of H. sabdariffa L. on cells in the oral cavity. |
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