• Users Online: 309
  • Print this page
  • Email this page
Year : 2021  |  Volume : 5  |  Issue : 1  |  Page : 42-46

Cytotoxicity of red fruit ethyl acetate extract (Pandanus conoideus lam.) on squamous cell carcinoma cell line (HSC-3)

1 Department of Oral Biology, Faculty of Dentistry, Maranatha Christian University, Bandung, Indonesia
2 Department of Periodontics, Faculty of Dentistry, Trisakti University, West Jakarta, Indonesia
3 Department of Biochemistry and Molecular Biology, Faculty of Dentistry, Trisakti University, West Jakarta, Indonesia

Correspondence Address:
Melanie S Djamil
Department of Biochemistry and Molecular Biology, Faculty of Dentistry, Trisakti University, West Jakarta
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/SDJ.SDJ_57_20

Rights and Permissions

Background: Oral squamous carcinoma is a malignancy of the head-and-neck area that comprises 90% of all oral cancers. Research continues to look for therapies with low concentrations of cytotoxicity to reduce morbidity in patients with tongue carcinoma. The red fruit plant (Pandanus conoideus Lam.) is believed to have anticancer activity because of its antiproliferation activity. The high antioxidant content in red fruit is able to ward off and break free radicals that carry carcinogen compounds. Red fruit ethyl acetate extract has the highest antioxidant activity compared with other fractions, such as water, chloroform, methanol, and n-hexane. Objective: This study sought to evaluate whether red fruit ethyl acetate extract is able to inhibit the growth of the Human Squamous Carcinoma (HSC-3) cell line with varying concentration levels and exposure times. Method: The HSC-3 cell line was treated with extract concentrations of 10 μg/mL, 20 μg/mL, and 40 μg/mL and exposure times of 6 and 12 h. Doxorubicin was used as a positive control, and dimethyl sulfoxide was used as a negative control. The percentage of viable HSC-3 cells was calculated through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay cytotoxicity test. All groups were statistically compared, and P < 0.05 was considered to be a statistically significant difference. Result: A concentration of 20 μg/mL with an exposure time of 6 h and a concentration of 10 μg/mL with an exposure time of 12 h showed a significant difference compared with the positive control of doxorubicin (P < 0.05). Conclusion: The results showed that the higher the concentration of red fruit ethyl acetate extract, the lower the percentage of viable HSC-3 cells.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded222    
    Comments [Add]    
    Cited by others 1    

Recommend this journal